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membrane potential fluorescent probe jc 1  (Beyotime)


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    Structured Review

    Beyotime membrane potential fluorescent probe jc 1
    Experimental validation. ( A ) qRT-PCR results for core gene mRNA expression (**P < 0.01, ***P < 0.001). ( B ) Western Blot bands for core proteins. ( C ) Oil Red O staining of control and NAFLD model HepG2 cells. ( D <t>)</t> <t>JC-1</t> fluorescence staining (red: aggregates, high potential; green: monomers, low potential).
    Membrane Potential Fluorescent Probe Jc 1, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 15087 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+potential+fluorescent+probe+jc+1/JC-1/pmc13032741-141-108-119
    Average 99 stars, based on 15087 article reviews
    membrane potential fluorescent probe jc 1 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Integrated Machine Learning and Multi-Omics Analysis Identifies Mitophagy-Related Core Genes and Mechanisms in Non-Alcoholic Fatty Liver Disease"

    Article Title: Integrated Machine Learning and Multi-Omics Analysis Identifies Mitophagy-Related Core Genes and Mechanisms in Non-Alcoholic Fatty Liver Disease

    Journal: Journal of Inflammation Research

    doi: 10.2147/JIR.S575586

    Experimental validation. ( A ) qRT-PCR results for core gene mRNA expression (**P < 0.01, ***P < 0.001). ( B ) Western Blot bands for core proteins. ( C ) Oil Red O staining of control and NAFLD model HepG2 cells. ( D ) JC-1 fluorescence staining (red: aggregates, high potential; green: monomers, low potential).
    Figure Legend Snippet: Experimental validation. ( A ) qRT-PCR results for core gene mRNA expression (**P < 0.01, ***P < 0.001). ( B ) Western Blot bands for core proteins. ( C ) Oil Red O staining of control and NAFLD model HepG2 cells. ( D ) JC-1 fluorescence staining (red: aggregates, high potential; green: monomers, low potential).

    Techniques Used: Biomarker Discovery, Quantitative RT-PCR, Expressing, Western Blot, Staining, Control, Fluorescence

    Related Articles

    Membrane:

    Article Title: Integrated Machine Learning and Multi-Omics Analysis Identifies Mitophagy-Related Core Genes and Mechanisms in Non-Alcoholic Fatty Liver Disease
    Article Snippet: The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen, USA) and 100 μg/mL penicillin-streptomycin (Suzhou New Cellmei Biotechnology Co., Ltd., China). .. The primary antibodies used were as follows: Anti-IGF1 Rabbit Polyclonal Antibody (IGF1, Catalog No. A11985; ABclonal Technology, China), Anti-MYH11 Rabbit Monoclonal Antibody (MYH11, Clone EPR5335; Abcam, Cambridge, UK), Anti-HYOU1 Rabbit Polyclonal Antibody (HYOU1, Catalog No. A1042; ABclonal Technology, Wuhan, China), Anti-SPATA18 Rabbit Polyclonal Antibody (SPATA18, Catalog No. A09906-1; ABclonal Technology, Wuhan, China), Anti-SCD (Stearoyl-CoA Desaturase) Rabbit Monoclonal Antibody (SCD, Catalog No. ab236868; Abcam, Cambridge, UK), and Anti-β-Actin Mouse Monoclonal Antibody (β-Actin, Catalog No. AC026; ABclonal Technology, Wuhan, China).Sodium palmitate (PA) and sodium oleate (OA) were obtained as a ready-made mixture (PA 6 mmol/L, OA 12 mmol/L; Catalog No. KC006) from Xi’an Kunchuang Technology Development Co., Ltd. (China).The mitochondrial membrane potential fluorescent probe JC-1 (Catalog No. C2006) was supplied by Beyotime Biotechnology (China). .. Carbonyl cyanide m-chlorophenyl hydrazone (CCCP; Product No. C2759) was purchased from Sigma-Aldrich (USA).



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    Image Search Results


    Experimental validation. ( A ) qRT-PCR results for core gene mRNA expression (**P < 0.01, ***P < 0.001). ( B ) Western Blot bands for core proteins. ( C ) Oil Red O staining of control and NAFLD model HepG2 cells. ( D ) JC-1 fluorescence staining (red: aggregates, high potential; green: monomers, low potential).

    Journal: Journal of Inflammation Research

    Article Title: Integrated Machine Learning and Multi-Omics Analysis Identifies Mitophagy-Related Core Genes and Mechanisms in Non-Alcoholic Fatty Liver Disease

    doi: 10.2147/JIR.S575586

    Figure Lengend Snippet: Experimental validation. ( A ) qRT-PCR results for core gene mRNA expression (**P < 0.01, ***P < 0.001). ( B ) Western Blot bands for core proteins. ( C ) Oil Red O staining of control and NAFLD model HepG2 cells. ( D ) JC-1 fluorescence staining (red: aggregates, high potential; green: monomers, low potential).

    Article Snippet: The primary antibodies used were as follows: Anti-IGF1 Rabbit Polyclonal Antibody (IGF1, Catalog No. A11985; ABclonal Technology, China), Anti-MYH11 Rabbit Monoclonal Antibody (MYH11, Clone EPR5335; Abcam, Cambridge, UK), Anti-HYOU1 Rabbit Polyclonal Antibody (HYOU1, Catalog No. A1042; ABclonal Technology, Wuhan, China), Anti-SPATA18 Rabbit Polyclonal Antibody (SPATA18, Catalog No. A09906-1; ABclonal Technology, Wuhan, China), Anti-SCD (Stearoyl-CoA Desaturase) Rabbit Monoclonal Antibody (SCD, Catalog No. ab236868; Abcam, Cambridge, UK), and Anti-β-Actin Mouse Monoclonal Antibody (β-Actin, Catalog No. AC026; ABclonal Technology, Wuhan, China).Sodium palmitate (PA) and sodium oleate (OA) were obtained as a ready-made mixture (PA 6 mmol/L, OA 12 mmol/L; Catalog No. KC006) from Xi’an Kunchuang Technology Development Co., Ltd. (China).The mitochondrial membrane potential fluorescent probe JC-1 (Catalog No. C2006) was supplied by Beyotime Biotechnology (China).

    Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing, Western Blot, Staining, Control, Fluorescence